Abstract
Estrogen receptor (ER)-beta mRNA splice variants have been identified in human breast tumors as well as normal human and mouse ovarian, uterine and mammary tissues. In both species transcripts deleted in exons 5 or 6, or 5 + 6 have been characterized by RT-PCR followed by cloning and sequencing. In mouse tissues an ER-beta transcript containing 54 nucleotides inserted in frame between exons 5 and 6 was identified. Interestingly, no equivalent of the mouse inserted transcript was detected in any of the four human tissues analyzed.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Breast / metabolism*
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Breast Neoplasms / genetics*
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Breast Neoplasms / metabolism
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Cloning, Molecular
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DNA Transposable Elements
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Estrogen Receptor beta
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Exons
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Female
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Genetic Variation*
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Humans
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Lactation
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Mammary Glands, Animal / metabolism
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Mice
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Molecular Sequence Data
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Ovary / metabolism*
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Polymerase Chain Reaction
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics*
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Receptors, Estrogen / biosynthesis
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Receptors, Estrogen / genetics*
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Recombinant Proteins / biosynthesis
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Sequence Deletion
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Uterus / metabolism*
Substances
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DNA Transposable Elements
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Estrogen Receptor beta
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RNA, Messenger
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Receptors, Estrogen
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Recombinant Proteins