DOC-2/hDab2, a candidate tumor suppressor gene involved in the development of gestational trophoblastic diseases

Oncogene. 1998 Jul 30;17(4):419-24. doi: 10.1038/sj.onc.1201955.

Abstract

Gestational trophoblastic diseases comprise a spectrum of interrelated diseases including partial mole, complete mole and gestational choriocarcinoma. Using reverse transcriptase PCR (RT-PCR) analysis, we identified higher levels of DOC-2/hDab2 expression in the normal trophoblast cells in culture than in choriocarcinoma cell lines. Subsequent study using immunohistochemistry showed high levels of DOC-2/hDab2 protein expression in normal trophoblast tissues but significantly lower levels of expression in gestational trophoblastic disease tissues, particularly in complete mole and choriocarcinoma. When DOC-2/hDab2 was transfected into the choriocarcinoma cell lines, Jar, JEG and BeWo, the stable transfectants showed significantly reduced growth rate in culture. These data suggest that down regulation of DOC-2/hDab2 may play an important role in the development of gestational trophoblastic diseases.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Adaptor Proteins, Vesicular Transport*
  • Amino Acid Sequence
  • Apoptosis Regulatory Proteins
  • Blotting, Western
  • Cell Division
  • Cell Line
  • Choriocarcinoma / genetics
  • Choriocarcinoma / metabolism
  • Choriocarcinoma / pathology
  • Coloring Agents
  • Female
  • Genes, Tumor Suppressor*
  • Humans
  • Hydatidiform Mole / genetics
  • Hydatidiform Mole / metabolism
  • Hydatidiform Mole / pathology
  • Hydatidiform Mole, Invasive / genetics
  • Hydatidiform Mole, Invasive / metabolism
  • Hydatidiform Mole, Invasive / pathology
  • Immunoenzyme Techniques
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • Pregnancy
  • Protein Biosynthesis
  • Proteins / genetics*
  • Tetrazolium Salts
  • Thiazoles
  • Transfection
  • Trophoblastic Neoplasms / genetics*
  • Tumor Cells, Cultured
  • Tumor Suppressor Proteins
  • Uterine Neoplasms / genetics*
  • Uterine Neoplasms / metabolism
  • Uterine Neoplasms / pathology

Substances

  • Adaptor Proteins, Signal Transducing
  • Adaptor Proteins, Vesicular Transport
  • Apoptosis Regulatory Proteins
  • Coloring Agents
  • DAB2 protein, human
  • Proteins
  • Tetrazolium Salts
  • Thiazoles
  • Tumor Suppressor Proteins
  • thiazolyl blue