Cyclooxygenase-2 expression in human adenocarcinoma cell line HT29 cl.19A

Anticancer Res. 1998 Jul-Aug;18(4A):2397-403.

Abstract

Background: Overexpression of cyclooxygenase-2 (COX-2) has been implicated in carcinogenesis of human colorectal cancer which is one of the leading types of cancer in Western countries.

Materials and methods: We analyzed the COX-2 expression and activity using RT-PCR, Western blot, immunocytochemistry, RP-HPLC and EIA analysis in 0% and 10% fetal bovine serum (FBS) cultured cells.

Results: HT 29 cl.19A cells exhibited a COX-2 expression called "constitutive" in the absence of FBS in culture media. This particular expression was not the result of a mutation of the HT29 cl.19A COX-2 gene promotor.

Conclusion: In our study, the human colon adenocarcinoma cell line, HT29 cl.19A, expressed COX-2 abnormally. This expression appeared to be at the same time inducible by the action of classical exogenous inducers such as FBS or interleukin-1 beta and "constitutive" if none of these compounds were present.

MeSH terms

  • Animals
  • Arachidonic Acid / metabolism
  • Cattle
  • Cell Division
  • Cloning, Molecular
  • Colorectal Neoplasms / enzymology
  • Culture Media
  • Culture Media, Serum-Free
  • Cyclooxygenase 2
  • DNA Primers
  • HT29 Cells
  • Humans
  • Immunohistochemistry
  • Isoenzymes / biosynthesis*
  • Isoenzymes / genetics
  • Membrane Proteins
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Prostaglandin-Endoperoxide Synthases / biosynthesis*
  • Prostaglandin-Endoperoxide Synthases / genetics
  • Recombinant Proteins / biosynthesis

Substances

  • Culture Media
  • Culture Media, Serum-Free
  • DNA Primers
  • Isoenzymes
  • Membrane Proteins
  • Recombinant Proteins
  • Arachidonic Acid
  • Cyclooxygenase 2
  • PTGS2 protein, human
  • Prostaglandin-Endoperoxide Synthases