No evidence for overexpression of the p53 protein and mutations in exons 4-9 of the p53 gene in a large family with adenomatous polyposis

Am J Gastroenterol. 1998 Sep;93(9):1524-6. doi: 10.1111/j.1572-0241.1998.00414.x.

Abstract

Objective: Familial adenomatous polyposis coli (FAP) is an autosomal dominant disease characterized by an early onset of numerous adenomatous polyps of the colon and a high risk of colon carcinoma. The role of the p53 gene in the multistage process of FAP is as yet poorly defined. In the present study, a large family with evidence of polyposis and colon cancer was screened for the mutations of the p53 gene and protein overexpression.

Methods: We examined p53 protein expression from individuals with immunohistochemical techniques using monoclonal antibody PAb1801. Polymerase chain reaction products of exons 4-9 of the p53 were examined from individuals by single strand, conformational polymorphism analysis.

Results: We could find no evidence of overexpression and mutations of the p53 in any lesion including adenomas and carcinomas.

Conclusion: We found that p53 gene alterations do not contribute to the genesis of adenoma or carcinoma of FAP patients for this large family examined.

MeSH terms

  • Adenomatous Polyposis Coli / genetics*
  • Adult
  • Exons / genetics*
  • Female
  • Genes, p53 / genetics*
  • Humans
  • Male
  • Mutation
  • Pedigree
  • Tumor Suppressor Protein p53 / metabolism*

Substances

  • Tumor Suppressor Protein p53