Abstract
Urokinase-type plasminogen activator (uPA) plays a central role in tissue remodeling and cell invasion. In the present study, we examined the expression of uPA in the prostate cancer cell lines LNCaP, DU-145 and PC-3. In contrast to DU-145 and PC-3, the androgen-responsive cell line LNCaP does not express uPA. However, seeding LNCaP cells on fibronectin-coated plates stimulated a low level of uPA expression which was further induced upon exposure of the cells to dihydrotestosterone (DHT). Concomitant with the expression of uPA, an androgen-regulated expression of uPA receptor (uPAR) was induced. These results suggest that the interaction of LNCaP cells with the extracellular matrix plays a dominant role in the androgen control of uPA and uPAR gene expression.
MeSH terms
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Dihydrotestosterone / pharmacology*
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Extracellular Matrix / physiology
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Extracellular Matrix Proteins / physiology
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Fibronectins / pharmacology*
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Gene Expression / drug effects
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Humans
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Male
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Neoplasm Invasiveness
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Neoplasm Proteins / genetics
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Neoplasm Proteins / metabolism*
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Prostatic Neoplasms / metabolism*
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Prostatic Neoplasms / pathology
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / metabolism*
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Receptors, Urokinase Plasminogen Activator
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Tumor Cells, Cultured
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Urokinase-Type Plasminogen Activator / genetics
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Urokinase-Type Plasminogen Activator / metabolism*
Substances
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Extracellular Matrix Proteins
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Fibronectins
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Neoplasm Proteins
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PLAUR protein, human
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Receptors, Cell Surface
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Receptors, Urokinase Plasminogen Activator
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Dihydrotestosterone
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Urokinase-Type Plasminogen Activator