Abstract
4-Hydroxy-2-keto-pentanoic acid aldolase from Escherichia coli was identified as a class I aldolase. The enzyme was found to be highly selective for the acetaldehyde acceptor but would accept alpha-ketobutyric acid or phenylpyruvic acid in place of the pyruvic acid carbonyl donor.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Acetaldehyde / metabolism
-
Aldehyde-Lyases / metabolism*
-
Butyrates / metabolism
-
Escherichia coli / enzymology*
-
Kinetics
-
Phenylpyruvic Acids / metabolism
-
Substrate Specificity
Substances
-
Butyrates
-
Phenylpyruvic Acids
-
alpha-ketobutyric acid
-
4-hydroxy-2-ketopentanoic acid aldolase
-
Aldehyde-Lyases
-
Acetaldehyde
-
phenylpyruvic acid