Abstract
Primer extension analysis and RNase protection assays revealed the identity of glucose 6-phosphatase gene transcripts in both the insulinoma cell line INS-1 and hepatic cells. In transient transfection assays of INS-1 cells, using constructs between the human glucose 6-phosphatase gene promoter and a luciferase reporter gene, the reporter gene activity was induced by dexamethasone and dibutyryl cAMP. Furthermore, the promoter was regulated by the glucose concentration in the medium. This effect was dependent on glucose metabolism. The data indicated that glucose 6-phosphatase gene transcription is regulated in a similar way in the insulinoma cell line and in liver.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bucladesine / pharmacology
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Calcium-Calmodulin-Dependent Protein Kinases / antagonists & inhibitors
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Dexamethasone / pharmacology
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Enzyme Induction
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Genes, Reporter
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Glucose / pharmacology*
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Glucose-6-Phosphatase / biosynthesis*
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Glucose-6-Phosphatase / genetics
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Humans
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Imidazoles / pharmacology
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Insulinoma
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Islets of Langerhans / drug effects*
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Liver / metabolism
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Luciferases / biosynthesis
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Luciferases / genetics
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Mitogen-Activated Protein Kinases*
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Promoter Regions, Genetic
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Pyridines / pharmacology
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Transcription, Genetic
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Tumor Cells, Cultured
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p38 Mitogen-Activated Protein Kinases
Substances
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Imidazoles
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Pyridines
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Bucladesine
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Dexamethasone
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Luciferases
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Calcium-Calmodulin-Dependent Protein Kinases
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Mitogen-Activated Protein Kinases
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p38 Mitogen-Activated Protein Kinases
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Glucose-6-Phosphatase
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Glucose
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SB 203580