Reactome | CAD hexamer dehydrates N-carb-L-Asp to (S)-DHO

CAD hexamer dehydrates N-carb-L-Asp to (S)-DHO

Stable Identifier

R-HSA-73571

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

Synonyms

N-carbamoyl L-aspartate + H+ <=> (S)-dihydroorotate + H2O, condensation of N-Carbamoyl-L-aspartate to form (S)-Dihydroorotate (ring closure)

ReviewStatus

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CAD hexamer dehydrates N-carb-L-Asp to (S)-DHO

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The synthesis of dihydroorotate from N-carbamoyl L-aspartate is catalyzed by the dihydroorotase activity of cytosolic trifunctional CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase) protein. This activity has not been directly demonstrated in experimental studies of the purified human protein, but has been inferred from the behavior of the purified hamster protein and the high degree of sequence similarity between the cloned hamster and human genes (Iwahana et al. 1996). Also on the basis of this similarity, the active human protein is annotated as a hexamer (Lee et al. 1985).

Literature References

PubMed ID	Title	Journal	Year
8619816	Molecular cloning of a human cDNA encoding a trifunctional enzyme of carbamoyl-phosphate synthetase-aspartate transcarbamoylase-dihydroorotase in de Novo pyrimidine synthesis Ii, S, Yoshimoto, K, Iwahana, H, Takahashi, Y, Itakura, M, Yamaoka, T, Kondo, M, Moritani, M, Fujimura, H	Biochem Biophys Res Commun	1996