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temozolomide |
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CHEBI:72564 |
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An imidazotetrazine that is 3,4-dihydroimidazo[5,1-d][1,2,3,5]tetrazine which is substituted at positions 3, 4, and 8 by methyl, oxo, and carboxamide groups, respectively. A prodrug for MTIC (5-(3-methyltriaz-1-en-1-yl)-1H-imidazole-4-carboxamide, formed by spontaneous hydrolysis of temozolomide in the body), it is used as an oral alkylating agent for the treatment of newly diagnosed malignant glioblastoma multiforme (concomitantly with radiotherapy) and malignant melanoma. |
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This entity has been manually annotated by the ChEBI Team.
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ChemicalBook:CB9454820, eMolecules:530768, Selleckchem:pmsf-phenylmethylsulfonyl-fluoride, ZINC000008220691 |
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SDF
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In biochemistry, phenylmethylsulfonyl fluoride (PMSF) is a serine protease inhibitor (serine hydrolase inactivator) commonly used in the preparation of cell lysates. PMSF does not inactivate all serine proteases. The effective concentration of PMSF is between 0.1 - 1 mM. The half-life is short in aqueous solutions (110 min at pH 7, 55 min at pH 7.5, and 35 min at pH 8, all at 25 °C). At 4˚C, pH 8, PMSF is almost completely degraded after 1 day. Stock solutions are usually made up in anhydrous ethanol, isopropanol, or corn oil and diluted immediately before use.
PMSF reacts specifically with the active site serine residue in serine hydrolases. It does not bind to any other serine residues in the protein. This is a result of the hyperactivity of that serine residue caused by the specific environmental conditions in the enzyme's active site (catalytic triad). Because PMSF bonds covalently to the enzyme, the complex can be viewed by X-ray crystallography; it can therefore be used as a chemical label to identify an essential active site serine in an enzyme.
Enzyme(active)Ser-O-H + F-SO2CH2C6H5 → EnzymeSer-O-SO2CH2C6H5 + HF
Serine protease + PMSF → Irreversible enzyme-PMS complex + HF
The median lethal dose between 150–215 mg/kg (acetylcholine esterase inactivator). PMSF should be handled only inside a fume hood and while wearing gloves. DMSO is sometimes recommended as solvent for stock solutions, but should not be used as it makes intact skin permeable to PMSF. |
Read full article at Wikipedia
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InChI=1S/C6H6N6O2/c1-11-6(14)12-2-8-3(4(7)13)5(12)9-10-11/h2H,1H3,(H2,7,13) |
BPEGJWRSRHCHSN-UHFFFAOYSA-N |
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alkylating agent
Highly reactive chemical that introduces alkyl radicals into biologically active molecules and thereby prevents their proper functioning. It could be used as an antineoplastic agent, but it might be very toxic, with carcinogenic, mutagenic, teratogenic, and immunosuppressant actions. It could also be used as a component of poison gases.
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antineoplastic agent
A substance that inhibits or prevents the proliferation of neoplasms.
prodrug
A compound that, on administration, must undergo chemical conversion by metabolic processes before becoming the pharmacologically active drug for which it is a prodrug.
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View more via ChEBI Ontology
3-methyl-4-oxo-3,4-dihydroimidazo[5,1-d][1,2,3,5]tetrazine-8-carboxamide
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temozolomida
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WHO MedNet
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témozolomide
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WHO MedNet
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temozolomide
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ChemIDplus
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temozolomidum
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WHO MedNet
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3,4-dihydro-3-methyl-4-oxoimidazo(5,1-d)-1,2,3,5-tetrazine-8-carboxamide
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ChemIDplus
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3,4-dihydro-3-methyl-4-oxoimidazo(5,1-d)-as-tetrazine-8-carboxamide
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ChemIDplus
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3-methyl-4-oxo-3,4-dihydroimidazo(5,1-d)(1,2,3,5)tetrazine-8-carboxamide
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ChemIDplus
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8-carbamoyl-3-methylimidazo(5,1-d)-1,2,3,5-tetrazin-4(3H)-one
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ChemIDplus
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BRN 5547136
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ChemIDplus
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CCRG 81045
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ChemIDplus
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CCRG-81045
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ChemIDplus
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CCRIS 8996
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ChemIDplus
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M & B 39831
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ChemIDplus
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MB 39831
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ChemIDplus
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M&B 39831
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ChemIDplus
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methazolastone
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ChemIDplus
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NSC 362856
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ChemIDplus
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Sch 52365
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ChemIDplus
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TMZ
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ChEBI
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Temodal
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KEGG DRUG
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Temodar
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ChEBI
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5547136
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Reaxys Registry Number
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Reaxys
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85622-93-1
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CAS Registry Number
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ChemIDplus
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22680781
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PubMed citation
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Europe PMC
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22818211
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PubMed citation
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Europe PMC
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23246370
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PubMed citation
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Europe PMC
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23254891
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PubMed citation
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Europe PMC
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23293540
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PubMed citation
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Europe PMC
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23335050
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PubMed citation
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Europe PMC
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23362460
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PubMed citation
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Europe PMC
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23377829
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PubMed citation
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Europe PMC
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23385995
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PubMed citation
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Europe PMC
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23389760
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PubMed citation
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Europe PMC
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