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| Status |
Public on Jan 22, 2019 |
| Title |
Principles and characteristics of the WRKY regulatory network of early MAMP-triggered immunity [ChIP-Seq] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
During microbe-associated molecular pattern (MAMP)-triggered immunity (MTI) more than 5000 Arabidopsis genes are significantly altered in their expression and the question arises, how such an enormous reprogramming of the transcriptome can be regulated in a safe and robust manner? For the WRKY transcription factors, which are important regulators not only of this defense response, it appears that they act in a complex regulatory sub-network rather than in a linear fashion, which is much more vulnerable to gene function loss either by pathogen-derived effectors or by mutations. In this study we employed RNA-seq, mass spectrometry and ChIP-seq to find evidence for and uncover principles and characteristics of this network.
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| Overall design |
For each of two biological replicates we analysed 4 different conditions: plants of WT and wrky18 wrky40 wrky33 triple mutant genotype, each sampled at 0 h (untreated) and 2 h flg22 treatment. For each condition the IP was perfomred with an anti-allWrky antibody and input DNA served as negative control for the peak calling.
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| Contributor(s) |
Birkenbihl RP, Somssich IE, Kracher B |
| Citation(s) |
30044528 |
| Submission date |
Jan 12, 2018 |
| Last update date |
Jan 24, 2019 |
| Contact name |
Barbara Kracher |
| E-mail(s) |
kracher@mpipz.mpg.de
|
| Organization name |
Max Planck Institute for Plant Breeding Research
|
| Department |
Plant-Microbe Interactions
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| Street address |
Carl-von-Linné-Weg 10
|
| City |
Cologne |
| ZIP/Postal code |
50829 |
| Country |
Germany |
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| Platforms (1) |
| GPL17639 |
Illumina HiSeq 2500 (Arabidopsis thaliana) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE109151 |
Principles and characteristics of the WRKY regulatory network of early MAMP-triggered immunity |
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| Relations |
| BioProject |
PRJNA429782 |
| SRA |
SRP129015 |
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