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| Status |
Public on Aug 19, 2018 |
| Title |
Genome-wide maps of LDL1pro::LDL1:GFP in 14 days old arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report the application of single-molecule-based sequencing technology for high-throughput profiling of H3K4 demethylase LDL1 in 14 days old arabidopsis. By obtaining sequence from chromatin immunoprecipitated DNA, we generated genome-wide LDL1-binding maps of 14 days old arabidopsis. To reveal bound genes by LDL1, chimeric protein LDL1-GFP was expressed under LDL1 promoter in ldl1 (LDL1pro:LDL1:GFP/ ldl1). ChIP was performed using anti-GFP antibody (ab290; ABCAM), and ChIP DNA were analyzed by Illumina HiSeq 2500.
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| Overall design |
Chromatin immunoprecipitation was performed for LDL1pro::LDL1:GFP-expressing Arabidopsis. ChIP DNA was analyzed by Illumina HiSeq 2500.
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| Contributor(s) |
Hung F, Wu K |
| Citation(s) |
30124938 |
| Submission date |
Aug 02, 2018 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Fu-Yu Hung |
| E-mail(s) |
clautus@mac.com
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| Organization name |
NTU, IPB
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| Street address |
Institute of Plant Biology, National Taiwan University
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| City |
Taipei |
| ZIP/Postal code |
10617 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL17639 |
Illumina HiSeq 2500 (Arabidopsis thaliana) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA484111 |
| SRA |
SRP156129 |
