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Series GSE132824 Query DataSets for GSE132824
Status Public on Jun 18, 2019
Title Transcriptome profiling of abiotic responses to heat, cold, salt, and osmotic stress of Capsicum annuum L.
Organism Capsicum annuum
Experiment type Expression profiling by high throughput sequencing
Summary We sought to investigate the scope of transcriptome analyses of peppers subjected to four major environment stresses (heat, cold, drought, and salinity). For this, at the six-true-leaf stage, plants were subjected to a temperature of 10°C or 40°C to mimic cold or heat stress, respectively. For salinity stress, plants were treated with 50 mL of a 400 mM NaCl solution; for osmotic stress, the peppers were treated with 50 mL of 400 mM mannitol. For RNA-seq library construction, the third or fourth leaves from four plants were harvested per replicate at 0, 3, 6, 12, 24, and 72 h after treatment. A total of 204.68 Gb of transcriptome data were generated using transcriptome analysis pipelines consisting of quality control, quantification, and differential gene expression analyses. A hierarchical clustering of gene expression data were used to infer the quality of the RNA-seq data and the characteristics of samples in each treatment. The extensive transcriptome data obtained will provide valuable information for future studies of crops exposed to abiotic stresses.
 
Overall design RNA-Seq profiling of abiotic responses to heat, cold, salt, and osmotic stress in Capsicum annuum L. leaves
 
Contributor(s) Kang W, Sim Y, Koo N, Nam J, Lee J, Kim N, Kim Y, Yeom S
Citation(s) 31932587
BioProject PRJNA525913
Submission date Jun 17, 2019
Last update date Jan 27, 2020
Contact name Seon-In Yeom
E-mail(s) sunin78@gnu.ac.kr
Organization name Gyeongsang National University
Street address 501, Jinjou-daero
City Jinju
ZIP/Postal code 52828
Country South Korea
 
Platforms (1)
GPL26789 Illumina HiSeq 2500 (Capsicum annuum)
Samples (78)
GSM3893035 Leaves, Abio-mock-0h-1
GSM3893036 Leaves, Abio-mock-0h-2
GSM3893037 Leaves, Abio-mock-0h-3
Relations
SRA SRP187794

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE132824_Abiotic_RNA-Seq.Readcount.txt.gz 2.3 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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