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| Status |
Public on Apr 28, 2020 |
| Title |
GCN5 modulates salicylic acid homeostasis by regulating H3K14ac levels at the 5ʹ and 3ʹ ends of its target genes |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The modification of histones by acetyl groups has a key role in the regulation of chromatin structure and transcription. The Arabidopsis thaliana histone acetyltransferase GCN5 regulates histone modifications as part of the Spt-Ada-Gcn5 Acetyltransferase (SAGA) transcriptional coactivator complex. GCN5 was previously shown to acetylate lysine 14 of histone 3 (H3K14ac) in the promoter regions of its target genes; however, its binding did not systematically correlate with gene activation and the mechanism by which GCN5 controls transcription thus remained unclear. To gain insight into GCN5 function, we fine-mapped its genome-wide binding sites and explored the effect of GCN5 loss-of-function on the expression of its target genes, finding that GCN5 has a dual role in the regulation of H3K14ac levels in their 5ʹ and 3ʹ ends. We found that the gcn5 mutation leads to a genome-wide reduction of H3K14ac in the 5ʹ end of some of the GCN5 targets, a phenomenon associated to their down-regulated in the gcn5 mutant. By contrast, an increase of H3K14ac in the 3ʹ end was observed in GCN5 targets that are up-regulated in the gcn5 mutant, indicating that this protein plays a dual role in the control of H3K14ac levels and in the regulation of transcription. Furthermore, changes in H3K14ac levels in the gcn5 mutant correlated with changes in H3K9ac in a genome-wide fashion at both 5ʹ and 3ʹ ends, providing evidence for a molecular link between the deposition of these two histone modifications. Finally, we show that GCN5 participates in responses to biotic stress by repressing salicylic acid (SA) accumulation and SA-mediated immunity, highlighting the role of this protein in the regulation of the crosstalk between diverse developmental and stress-responsive physiological programs.
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| Overall design |
Examination of 1 histone modification (H3K14ac) in two allelic mutants of GCN5 and wild-type control and target analysis of GCN5-GFP protein (2 replicates)
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| Contributor(s) |
Kim S, Piquerez S, Ramirez-Prado JS, Veluchamy A, Latrasse D, Manza-Mianza D, Brik-Chaouche R, Rodriguez-Granados NY, Concia L, Blein T, Bendahmane A, Bergounioux C, Crespi M, Mahfouz MM, Raynaud C, Hirt H, Ntoukakis V, Benhamed M |
| Citation(s) |
32396165 |
| Submission date |
Sep 16, 2019 |
| Last update date |
Jun 22, 2020 |
| Contact name |
Déborah MANZA MIANZA |
| E-mail(s) |
deborah.manza-mianza@ips2.universite-paris-saclay.fr
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| Organization name |
Institut des Sciences des Plantes - Paris Saclay
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| Lab |
Chromosome Dynamics
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| Street address |
630 Rue Noetzlin
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| City |
Gif-sur-Yvette |
| ZIP/Postal code |
91190 |
| Country |
France |
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| Platforms (1) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA565696 |
| SRA |
SRP221724 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE137474_RAW.tar |
4.9 Gb |
(http)(custom) |
TAR (of BED, BIGWIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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