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| Status |
Public on Apr 11, 2022 |
| Title |
Jasmonates and Histone deacetylase 6 activate Arabidopsis genome-wide histone acetylation and methylation during the early acute stress response |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
To analyze the extent of the overlap between MeJA-induced transcripts and MeJA- induced histone acetylation, the expression profiles of WT untreated (WT), WT+MeJA, axe1-5 untreated and axe1-5+MeJA samples were analysed using the catalog microarray (Agilent design ID:21169).
Whole A. thaliana seedlings of axe1-5 mutant and DR5 wild-type lines (Murfett et al 2001) were grown in vitro for 12 days on 1% Murashige and Skoog (MS) medium (Duchefa) supplemented with 1% sucrose, 0.8% phytoagar (Duchefa) and buffered to pH 5.8. Stratification of seeds was performed for 3 days at 4°C in the dark. Seeds were then grown with plates approximately 20° to vertical under 16h light/8h dark cycles for 12 days at 22°C and a light intensity of 100-120 µmol m-2s-1. Twelve-day-old in vitro grown whole seedlings were transferred to 50 µM MeJA or mock plates and sampled after 3 hours for microarray analysis. Expression profiles were analyzed using the catalog microarray (Agilent design ID:21169).
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| Overall design |
Total 8 samples were analyzed.
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| Contributor(s) |
Vincent S, Kim J, Pérez-Salamó I, To TK, Torii C, Ishida J, Tanaka M, Endo TA, Bhat P, Devlin P, Seki M, Devoto A |
| Citation(s) |
35399062 |
| Submission date |
Dec 08, 2020 |
| Last update date |
Apr 19, 2022 |
| Contact name |
Alessandra Devoto |
| E-mail(s) |
Alessandra.Devoto@rhul.ac.uk
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| Organization name |
Royal Holloway University of London
|
| Street address |
Egham Hill
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| City |
Egham |
| ZIP/Postal code |
TW20 0EX |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL12621 |
Agilent-021169 Arabidopsis 4 Oligo Microarray (V4) (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA683647 |
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