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| Status |
Public on Mar 02, 2012 |
| Title |
Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response (WT and G9a deficient MEFs) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Effective anti-viral immunity depends on the ability of infected cells or cells triggered with virus-derived nucleic acids to produce type I interferon (IFN), which activates transcription of numerous antiviral genes. However, disproportionately strong or chronic IFN expression is a common cause of inflammatory and autoimmune diseases. Here we describe an epigenetic mechanism that determines cell-type specific differences in IFN and IFN-stimulated gene expression in response to exogenous signals. We identify di-methylation of histone H3 at lysine 9 (H3K9me2) as a suppressor of IFN and IFN-inducible antiviral gene expression. We show that levels of H3K9me2 at IFN and IFN stimulated genes (ISG) correlate inversely with the scope and amplitude of IFN and ISG expression in fibroblasts and dendritic cells. Accordingly, genetic ablation or pharmacological inactivation of lysine methyltransferase G9a, which is essential for the generation of H3K9me2, resulted in phenotypic conversion of fibroblasts into highly potent IFN-producing cells and rendered these cells resistant to pathogenic RNA viruses. In summary, our studies implicate H3K9me2 and enzymes controlling its abundance as key regulators of innate antiviral immunity.
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| |
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| Overall design |
Examination of gene expression in response to PolyI:C in WT and G9a deficient MEFs
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| |
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| Contributor(s) |
Fang TC, Tarakhovsky A |
| Citation(s) |
22412156 |
| Submission date |
Oct 18, 2010 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Terry Fang |
| E-mail(s) |
tfang@rockefeller.edu
|
| Phone |
212-327-8265
|
| Fax |
212-327-8258
|
| Organization name |
Rockefeller University
|
| Street address |
1230 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (26)
|
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| This SubSeries is part of SuperSeries: |
| GSE24826 |
Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response |
|
| Relations |
| BioProject |
PRJNA133385 |
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