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| Status |
Public on Sep 25, 2017 |
| Title |
Cytosolic acetyl-CoA promotes histone acetylation predominantly at H3K27 in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Acetyl-Coenzyme A (acetyl-CoA) is a central metabolite and the acetyl source for protein acetylation, particularly histone acetylation that promotes gene expression. However, the effect of acetyl-CoA levels on histone acetylation status in plants remains unknown. Here, we show that malfunctioned cytosolic acetyl-CoA carboxylase1 (ACC1) in Arabidopsis leads to elevated levels of acetyl-CoA and promotes histone hyperacetylation predominantly at lysine 27 of histone H3 (H3K27). The increase of H3K27 acetylation (H3K27ac) is dependent on ATP-citrate lyase which cleaves citrate to acetyl-CoA in the cytoplasm, and requires histone acetyltransferase GCN5. A comprehensive analysis of the transcriptome and metabolome in combination with the genome-wide H3K27ac profiles of acc1 mutants, demonstrate the dynamic changes of H3K27ac, gene transcripts and metabolites occurring in the cell by the increased levels of acetyl-CoA. This study suggests that H3K27ac is an important link between cytosolic acetyl-CoA level and gene expression in response to the dynamic metabolic environments in plants.
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| Overall design |
Examination of 9 different histone modifications in WT and acc1-5 seedlings and of H3K27 acetylation in acla-1i acc1-5 before and after treating with β-estrodial.
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| Contributor(s) |
Chen C, Li C, Wang Y, Renaud J, Tian G, Kambhampati S, Saatian B, Nguyen V, Hannoufa A, Marsolais F, Yuan Z, Yu K, Austin RS, Liu J, Kohalmi SE, Wu K, Huang S, Cui Y |
| Citation(s) |
28947800 |
| Submission date |
Mar 23, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Chen Chen |
| E-mail(s) |
chenchen101@scbg.ac.cn
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| Organization name |
South China Botanical Garden, Chinese Academy of Science
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| Lab |
Dr. Chen Chen
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| Street address |
723 Xinke Rd.
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| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510650 |
| Country |
China |
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| Platforms (2) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
| GPL17639 |
Illumina HiSeq 2500 (Arabidopsis thaliana) |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA316129 |
| SRA |
SRP072236 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE79524_RAW.tar |
1014.7 Mb |
(http)(custom) |
TAR (of WIG) |
| GSE79524_Transcript_FPKM_tracking_in_WT_and_acc1-5.txt.gz |
1.7 Mb |
(ftp)(http) |
TXT |
| GSE79524_Transcript_differentrial_expression_testing_in_WT_and_acc1-5.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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