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| Status |
Public on Jun 13, 2017 |
| Title |
MAPK-triggered chromatin reprogramming by histone deacetylase in plant innate immunity |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
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| Summary |
Microbial-associated molecular patterns (MAMPs) activate several MAP Kinases (MAPKs), which are major regulators of the innate immune response in Arabidopsis that induce large-scale changes in gene expression. Here, we determined whether MAMP-triggered gene expression involves chromatin modifications at the chromosomal level. Our results show that histone acetylation and deacetylation are major regulators of MAMP-triggered gene expression and implicate the histone deacetylase HD2B in the reprogramming of defense gene expression and innate immunity. The MAPK MPK3 directly interacts with and phosphorylates HD2B, thereby regulating the intra-nuclear compartmentalization and function of the histone deacetylase. By studying a number of gene loci that undergo MAMP-dependent activation or repression, our data reveal a mechanistic model for how protein kinase signaling directly impacts chromatin reprogramming in plant defense.
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| Overall design |
Genome-wide correlation of H3K9ac modification and HD2B binding profile to transcription.
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| Contributor(s) |
Latrasse D, Jégu T, Li H, de Zelicourt A, Raynaud C, Legras S, Gust A, Samajova O, Veluchamy A, Rayapuram N, Ramirez-Prado JS, Kulikova O, Colcombet J, Bigeard J, Genot B, Bisseling T, Benhamed M, Hirt H |
| Citation(s) |
28683804 |
| Submission date |
Jun 12, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Alaguraj Veluchamy |
| E-mail(s) |
alaguraj.v@gmail.com
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| Organization name |
King Abdullah University of Science and Technology
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| Department |
BESE
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| Street address |
Thuwal
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| City |
Thuwal |
| ZIP/Postal code |
23955-6900 |
| Country |
Saudi Arabia |
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| Platforms (1) |
| GPL17639 |
Illumina HiSeq 2500 (Arabidopsis thaliana) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA390146 |
| SRA |
SRP109012 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE99936_HD2B_RPKM_RNAseq.txt.gz |
2.1 Mb |
(ftp)(http) |
TXT |
| GSE99936_RAW.tar |
122.7 Mb |
(http)(custom) |
TAR (of WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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