URL: http://antibodyregistry.org/AB_2200235
Proper Citation: (R and D Systems Cat# AF2085, RRID:AB_2200235)
Target Antigen: Human/Mouse Brachyury Affinity Purified Ab
Host Organism: goat
Clonality: polyclonal
Comments: vendor recommendations: IgG Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunohistochemistry, Western Blot; Immunohistochemistry; Immunocytochemistry; Western Blot
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We observed high levels of non-specific antibody staining, including for BRACHYURY and N-CADHERIN in the Rreb1-/- VE. Such non-specific staining is often observed in the VE of wild-type embryos prior to intercalation of the DE (Kwon et al., 2008; Morgani et al., 2018a), which has ben attributed to its extensive vacuolation. Thus, we hypothesized that there may be defects in DE intercalation in mutant embryos.
Source: Antibody Registry
