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Plasmid Name
RRID:Addgene_10878 RRID Copied  
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RRID:Addgene_10878
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Plasmid Information

URL: http://www.addgene.org/10878

Proper Citation: RRID:Addgene_10878

Insert Name: stuffer

Bacterial Resistance: Ampicillin

Defining Citation: PMID:16564017

Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin

Comments: This is the recommended vector for cloning and expressing new shRNA sequences. This plasmid is being used by The RNAi Consortium to produce their shRNA library http://www.broad.mit.edu/genome_bio/trc/ The 1.9kb stuffer can be released with AgeI and EcoRI and replaced with your shRNA sequence of choice. The link to the author's map shows the key features of this vector before the 1.9kb stuffer was cloned in. The link to the sequence shows the entire sequence with the stuffer sequence in capital letters. Also, see Addgene's pLKO.1 protocol http://www.addgene.org/plko on how to use the pLKO.1 vector. Plasmid grows more slowly than standard plasmids.

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Data and Source Information

Source: Addgene