URL: http://www.addgene.org/12457
Proper Citation: RRID:Addgene_12457
Insert Name: mutant TCF/LEF binding sites
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12699626
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4839; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: This is a CONTROL luciferase reporter with the TCF/LEF sites of Super8XTOPflash (construct M50, Addgene Plasmid #12456) being mutated. There are 6 mutated TCF/LEF binding sites that were cloned into the pGL3 vector (Promega), which contains a minimal promoter followed by a firefly luciferase open reading frame. This 134bp fragment was gene synthesized and cloned into the ASP718 and XMA-1 sites of the vector. Note: This plasmid was published as M51 Super 8x FOPFlash, but the plasmid actually contains 6 TCF/LEF sites.
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Source: Addgene