URL: http://www.addgene.org/179892
Proper Citation: RRID:Addgene_179892
Insert Name: RAB11A, member RAS oncogene family
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:35708998
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Other, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Comments: Here donor for integration of SFFV-T2A-Puromycine resistance-PolyA cassette (for knock out) to Rab11a locus This can be combined with knock in of DExCon module to be able to reversibly rescue Rab11a expression (from the second allele) on demand DExCon = Doxycycline-mediated endogenous gene Expression Control https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281. Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
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Source: Addgene