URL: http://www.addgene.org/18121
Proper Citation: RRID:Addgene_18121
Insert Name: Zsgreen
Organism: Anthozoa
Bacterial Resistance: Ampicillin
Defining Citation: PMID:18371425
Vector Backbone Description: Backbone Size:6900; Vector Backbone:pSICO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: HIV-Zsgreen was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Zsgreen. cDNA can be cloned into the MCS (NotI, EcoRI, HpaI, XbaI, SmaI, and BamHI sites) to enable bicistronic expression with Zsgreen.
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Source: Addgene