URL: http://www.addgene.org/24478
Proper Citation: RRID:Addgene_24478
Insert Name: SypHy
Organism: Rattus norvegicus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:16982422
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3952; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: The depositing lab notes that the Xba1 and Bcl1 unique restriction digestion sites could be methylated and blocked if the plasmid is grown or maintained in dam+ bacteria. Also note that in the Addgene-generated map below, the feature listed as "GFP(12-708)" is actually pH sensitive pHluorin. There is a mutation S446T in Synaptophysin ORF. This change does not have any effect in localizing the construct to the synaptic vesicle and may be a result of a SNP.
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Source: Addgene