URL: http://www.addgene.org/26106
Proper Citation: RRID:Addgene_26106
Bacterial Resistance: Kanamycin
Defining Citation: PMID:20541610
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5671; Vector Backbone:pET-28-a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Primers for LIC cloning: Upstream: add TACTTCCAATCCATG to the 5’ end (ATG in-frame with the desired coding sequence). Downstream: add TATCCACCTTTACTG to 5’ end of downstream primer; add termination codon, if necessary. Detailed cloning method available in the paper (Savitsky et al., J Struct Biol., 2010)
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Source: Addgene