URL: http://www.addgene.org/30127
Proper Citation: RRID:Addgene_30127
Bacterial Resistance: Ampicillin
Defining Citation: PMID:
Vector Backbone Description: Backbone Size:6145; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: This is an empty LIC vector derived from pcDNA3. It adds a GFP gene to the C-terminus of your protein of interest. GFP has a excitation max of 489 nm and an emission max of 510 nm. To clone into this vector, add the following tags to your PCR primers: LIC vKoz Forward tag 5’-TACTTCCAATCCAATGCCACC(ATG) LIC vGFP Reverse tag 5’-CTCCCACTACCAATGCC Do NOT include a stop codon with your reverse primer. T4-treat PCR with dCTP. Linearize vector with SspI, then T4-treat with dGTP. Can verify the presence of insert by digesting with HindIII and XbaI. For more information, please see our website: http://qb3.berkeley.edu/qb3/macrolab/
Expand AllWe found {{ ctrl2.mentions.total_count }} mentions in open access literature.
We have not found any literature mentions for this resource.
We are searching literature mentions for this resource.
Most recent articles:
{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})
A list of researchers who have used the resource and an author search tool
A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.
No rating or validation information has been found for pcDNA3 GFP LIC cloning vector (6D).
No alerts have been found for pcDNA3 GFP LIC cloning vector (6D).
Source: Addgene