URL: http://www.addgene.org/45605
Proper Citation: RRID:Addgene_45605
Insert Name: FRT-kan-FRT
Bacterial Resistance: Ampicillin and Kanamycin
Defining Citation: PMID:10829079
Vector Backbone Description: Backbone Marker:M. Koob (University of Wisconsin, Madison); Vector Backbone:pANTSγ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is a template plasmids for frt-flanked kan cassette. The kan cassette originally came from pCP15. pKD3 and pKD4 are identical except for the region between the FRT sites, so they create an identical scar that has stop codons in all six reading frames. As drawn in the map, this scar has an idealized ribosome binding site and start codon for downstream (rightward) gene expression.
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Source: Addgene