URL: http://www.addgene.org/62988
Proper Citation: RRID:Addgene_62988
Insert Name: hSpCas9-2A-Puro V2.0
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:24157548
Vector Backbone Description: Vector Backbone:PX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: NOTE: This is an improved version of Addgene Plasmid #48139. It has improved puromycin selection. The EcoRI site is not unique and flanks the puromycin cassette. For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/ The guide sequence is cloned into this plasmid using BbsI sites. The hU6-F primer (5'-GAGGGCCTATTTCCCATGATT-3') can be used to confirm the gRNA sequence after cloning into the plasmid. Please see the cloning protocol provided by the Zhang lab for more details.
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Source: Addgene