URL: http://www.addgene.org/63215
Proper Citation: RRID:Addgene_63215
Insert Name: His-T7-eGFP
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:22639380
Vector Backbone Description: Backbone Marker:Jakob Reiser; Backbone Size:10680; Vector Backbone:pNL-eGFP/CEF; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Lentiviral vector with high transduction efficiency. Expression from the CMV-EF1α hybrid (CEF) promoter is very stable in mammalian cells. The lentivirus contains an SV40 origin 5’ to the eGFP gene. This influences gene targeting rates and the preferred strand for gene targeting and recombineering in cells expressing the SV40 T-antigen.
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Source: Addgene