URL: http://www.addgene.org/82416
Proper Citation: RRID:Addgene_82416
Insert Name: GFP
Organism: Other
Bacterial Resistance: Ampicillin
Defining Citation: PMID:28202515
Vector Backbone Description: Backbone Marker:Feng Zhang; Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: sgRNA of choice is cloned into vector using Golden Gate assembly, with BsmBI restriction enzyme (as per resource information on Addgene plasmid #52961 by Feng Zhang). Note: This plasmid does not contain a 2KB stuffer sequence between the BsmBI sites. sgRNA sequencing primer is as follows: 5'-TACGTGACGTAGAAAGTA
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Source: Addgene