URL: http://mendel.imp.ac.at/sat/gpi/gpi_server.html
Proper Citation: big-PI Predictor (RRID:SCR_001599)
Description: Prediction tool locating potential GPI-modification sites in precursor sequences applied for large-scale protein sequence database searches. The composite prediction function (with separate parametrization for metazoan and protozoan proteins) consists of terms evaluating both amino acid type preferences at sequence positions near a supposed omega-site as well as the concordance with general physical properties encoded in multi-residue correlation within the motif sequence. The latter terms are especially successful in rejecting non-appropriate sequences from consideration. The algorithm has been validated with a self-consistency and two jack-knife tests for the learning set of fully annotated sequences from the SWISS-PROT database as well as with a newly created database big-Pi (more than 300 GPI-motif mutations extracted from original literature sources). The accuracy of predicting the effect of mutations in the GPI sequence motif was above 83 %.
Abbreviations: big-PI Predictor
Synonyms: big-PI Predictor: GPI Modification Site Prediction
Resource Type: data analysis service, analysis service resource, production service resource, service resource
Defining Citation: PMID:10497036, PMID:10871885
Keywords: prediction, glycoprotein, protein, glycosylphosphatidylinositol, genome annotation, target selection, FASEB list
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