URL: http://www.wormbase.org/db/get?name=WBStrain00024080
Proper Citation: RRID:WB-STRAIN:WBStrain00024080
Description: Caenorhabditis elegans with name ifg-1(cxTi9279)II; bcIs39 V. from WB.
Species: Caenorhabditis elegans
Synonyms: ifg-1(cxTi9279)II; bcIs39 V.
Notes: bcIs39 [lim-7p::ced-1::GFP and lin-15(+)] V. Temperature-sensitive germ cell apoptosis leading to infertility at 25 C. Apoptosing germ cells are decorated by CED-1::GFP in the gonad. Loss of p170 form of IFG-1 (but not p130 IFG-1) confirmed by Western blot. Escaping eggs are fertilized and embryonic lethal. Mos-1 transposon insertion previously described as ifg-1::mos-1(cxP9279)|"bcIs39 [lim-7p::ced-1::GFP and lin-15(+)] V. Temperature-sensitive germ cell apoptosis leading to infertility at 25 C. Apoptosing germ cells are decorated by CED-1::GFP in the gonad. Loss of p170 form of IFG-1 (but not p130 IFG-1) confirmed by Western blot. Escaping eggs are fertilized and embryonic lethal. Mos-1 transposon insertion previously described as ifg-1::mos-1(cxP9279)II; confirmed by triple primer PCR with 5-ACCAAACTGGGCAAACAAAG-3, 5-GCTCAATTCGCGCCAAACTATG-3, and 5-CTTCCTGAAATTTGGTTTAACAGT-3. Homozygous ifg-1::mos yields only 444 bp product. Outcrossed heterozygotes yield both 353 bp (wild type ifg-1) and 444 bp products."|"Made_by: Melissa A Henderson"
Affected Gene: WBGene00002066(ifg-1)
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Source: Integrated Animals
Source Database: WormBase (WB)