URL: http://www.wormbase.org/db/get?name=WBStrain00041080
Proper Citation: RRID:WB-STRAIN:WBStrain00041080
Description: Escherichia coli with name F-, mcrA, mcrB, IN(rrnD-rrnE)1, rnc14::Tn10(DE3 lysogen: lavUV5 promoter -T7 polymerase) (IPTG-inducible T7 polymerase) (RNAse III minus). This strain grows on LB or 2XYT plates. This strain is tetracycline resistant. Researchers using this strain should test for expression by transforming in one of the plasmids from the Fire Vector Kit (1999) (pLT76, e.g.) using standard CaCl2 transformation techniques. Biosafety Level: BSL-1. from WB.
Species: Escherichia coli
Synonyms: F-, mcrA, mcrB, IN(rrnD-rrnE)1, rnc14::Tn10(DE3 lysogen: lavUV5 promoter -T7 polymerase) (IPTG-inducible T7 polymerase) (RNAse III minus). This strain grows on LB or 2XYT plates. This strain is tetracycline resistant. Researchers using this strain should test for expression by transforming in one of the plasmids from the Fire Vector Kit (1999) (pLT76, e.g.) using standard CaCl2 transformation techniques. Biosafety Level: BSL-1.
Notes: Bacteria. Genotype: F-, mcrA, mcrB, IN(rrnD-rrnE)1, rnc14::Tn10(DE3 lysogen: lacUV5 promoter -T7 polymerase) (IPTG-inducible T7 polymerase) (RNAse III minus). This strain grows on LB or 2XYT plates. This strain is tetracycline resistant. Researchers using this strain should test for expression by transforming in one of the plasmids from the Fire Vector Kit (1999) (pLT76, e.g.) using standard CaCl2 transformation techniques. Biosafety Level: BSL-1.|"Merged the Strain HT115 into here as it was a duplicate/incorrect addition."|"Non-elegans Strain"|"WBStrain provided so WBPaper00060161 paper added based on AFP_Strain data."|"WBStrain provided so WBPaper00060223 paper added based on AFP_Strain data."|"WBStrain provided so WBPaper00060797 paper added based on AFP_Strain data."|"WBStrain provided so WBPaper00061698 paper added based on AFP_Strain data."|"WBStrain provided so WBPaper00061805 paper added based on AFP_Strain data."|"WBStrain provided so WBPaper00061894 paper added based on AFP_Strain data."|"WBStrain provided so WBPaper00062059 paper added based on AFP_Strain data."|"WBStrain provided so WBPaper00062146 paper added based on AFP_Strain data."
Affected Gene: EMPTY
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No rating or validation information has been found for HT115(DE3).
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Source: Integrated Animals
Source Database: WormBase (WB)