Proper Citation: Perkin Elmer UltraView ERS (RRID:SCR_020405)
Description: PerkinElmer UltraVIEW system (PerkinElmer Life Sciences Inc., MA, USA) is a Yokogawa (Yokogawa Corp. Japan) Nipkow Spinning Disk Confocal System. It uses a spinning disk with multiple pinholes to achieve confocality (e.g. the rejection of out-of-focus light). Emission light from the sample passes through the pinholes to generate a confocal image of the sample that can be detected with an EMCCD (Electron Multiplification Charge-Coupled Device) camera. Spinning disk confocal systems have 2 main advantages over conventional Laser Scanning Confocal Microscopes (LSCM): Higher imaging speed: up to 360frames/sec compared to 0.5-1 frames per second in a LSC and Lower photo-toxicity: around 5 times less than a LSCM, probably due to the fact that the system splits the laser light into thousands of minibeams.
Resource Type: instrument resource
Keywords: Perkin Elmer, Confocal Spinning Disk Microscope, Instrument Equipment, USEDit
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