Examples: histone, BN000065

Project: PRJEB47776

Induced pluripotent stem cell (iPSC) derived organoid systems provide models to study human organ development. Single-cell transcriptome sequencing enables highly-resolved descriptions of cell state heterogeneity within these systems and computational methods can reconstruct developmental trajectories. However, new approaches are needed to directly measure lineage relationships in these systems. Here we establish an inducible dual channel lineage recorder, iTracer, that couples reporter barcodes, inducible CRISPR/Cas9 scarring, and single-cell transcriptomics to analyze state and lineage relationships in iPSC-derived systems. This data set include the iTracer data of 12 cerebral organoids.

Secondary Study Accession:
ERP132077
Study Title:
Lineage recording in human cerebral organoids (whole organoids)
Center Name:
European Bioinformatics Institute;ETH Zurich;D-BSSE
Study Name:
Lineage recording in human cerebral organoids (whole organoids)
Broker Name:
ArrayExpress
ENA-FIRST-PUBLIC:
2021-11-03
ENA-LAST-UPDATE:
2021-11-03
General