Project: PRJNA629752
Although most SARS-CoV-2-infected individuals experience mild COVID-19, some patients suffer from severe COVID-19, which is accompanied by acute respiratory distress syndrome and systemic inflammation. To identify factors driving severe progression of COVID-19, we performed single-cell RNA-seq using peripheral blood mononuclear cells (PBMCs) obtained from healthy donors, patients with mild or severe COVID-19, and patients with severe influenza. Patients with COVID-19 exhibited hyper-inflammatory signatures across all types of cells among PBMCs, particularly upregulation of the TNF/IL-1beta-driven inflammatory response as compared to severe influenza. In classical monocytes from patients with severe COVID-19, type I IFN response co-existed with the TNF/IL-1beta-driven inflammation, and this was not seen in patients with milder COVID-19 infection. Based on this, we propose that the type I IFN response exacerbates inflammation in patients with severe COVID-19 infection. Overall design: Single-cell RNA sequencing performed with PBMC of 5 flu, 11 COVID-19 patients and 4 healthy controls. The suffix of each barcode sequence represents patient information. '-1' - 'Sample1' - 'nCoV 1 scRNA-seq' '-2' - 'Sample2' - 'nCoV 2 scRNA-seq' '-3' - 'Sample3' - 'Flu 1 scRNA-seq' '-4' - 'Sample4' - 'Flu 2 scRNA-seq' '-5' - 'Sample5' - 'Normal 1 scRNA-seq' '-6' - 'Sample6' - 'Flu 3 scRNA-seq' '-7' - 'Sample7' - 'Flu 4 scRNA-seq' '-8' - 'Sample8' - 'Flu 5 scRNA-seq' '-9' - 'Sample9' - 'nCoV 3 scRNA-seq' '-10' - 'Sample10' - 'nCoV 4 scRNA-seq' '-11' - 'Sample11' - 'nCoV 5 scRNA-seq' '-12' - 'Sample12' - 'nCoV 6 scRNA-seq' '-13' - 'Sample13' - 'Normal 2 scRNA-seq' '-14' - 'Sample14' - 'Normal 3 scRNA-seq' '-15' - 'Sample15' - 'nCoV 7 scRNA-seq' '-16' - 'Sample16' - 'nCoV 8 scRNA-seq' '-17' - 'Sample17' - 'nCoV 9 scRNA-seq' '-18' - 'Sample18' - 'nCoV 10 scRNA-seq' '-19' - 'Sample19' - 'Normal 4 scRNA-seq' '-20' - 'Sample20' - 'nCoV 11 scRNA-seq'
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