Examples: histone, BN000065

Project: PRJNA702442

Regenerative medicine relies on basic research to find safe and useful outcomes that are only practical when cost-effective. The human eyeball requires the retinal pigment epithelium (RPE) for support and maintenance that interfaces the neural retina and the choroid at large. Nearly 200 million people suffer from age-related macular degeneration (AMD), a blinding multifactor genetic disease among other retinal pathologies related to RPE degradation. Recently, autologous pluripotent stem cell-derived RPE cells were prohibitively expensive due to time, therefore we developed a new simplified cell reprogramming system. We stably induced RPE-like cells (iRPE) from human fibroblasts by conditional overexpression of broad plasticity and lineage-specific pioneering transcription factors and treated some with nicotinamide and chetomin (iRPENC). iRPE cells showed features of modern RPE benchmarks and significant in-vivo integration in transplanted chimeric hosts. Herein, we provide the single-cell RNA (scRNA) sequencing profiling of the foreskin fibroblasts, iRPE, iRPENC, and model iPSC.RPE cells. Overall design: Cell culture samples were prepared as approximately 3,000 single cells and then sampled for scRNA-seq 10x Genomics 5' (v1.0 Chemistry) VDJ kit library construction with standard protocol for 5' Gene Expressoin libraries that skips Steps 4&5. Libraries were sequenced with paired-end reads on Illumina HiSeq X at one library per lane and then mapped to a custom reference human genome (hg38) using Cell Ranger (Version 3.1). related study: https://doi.org/10.1101/2020.07.27.215103

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