Hydrosalpinx is common cause of tubal factor infertility in women in which the fallopian tube becomes blocked, fills with fluid, and consequently is nonfunctional. Single cell RNA-seq analysis has provided an unparalleled approach to characterize tissue types and explore pathologies in unbiased manner. Here we use scRNA-seq to define and compare major and rare cell types of the human fallopian tube in the healthy and hydrosalpinx disease state. Overall design: Characterizing cellular composition and heterogeneity of healthy state and hydrosalpinx disease state of the human fallopian tubes using 10x scRNA-seq analysis