Project: PRJNA437966
Despite effective treatment, HIV can persist in latent reservoirs, which represent a major obstacle towards HIV eradication. Targeting and reactivating latent cells is challenging due to the heterogeneous nature of HIV infected cells. Here, we used a primary model of HIV latency and single-cell RNA sequencing to characterize transcriptional heterogeneity during HIV latency and reactivation. Our analysis identified transcriptional programs leading to successful reactivation of HIV expression. We further validated our results using primary CD4+ T cells isolated from HIV+ individuals. Overall design: Human primary CD4+ T-cells were infected, cultured, and maintained in a resting, latent phenotype in order to generate a primary model of HIV latency. Latently infected cells were either left untreated, or exposed to SAHA or TCR stimulation, followed by single-cell isolation and single-cell RNA-seq (scRNA-Seq) analysis. Bulk RNA-Seq experiments were also performed as control. To validate the observed cellular heterogeneity in the primary model of HIV latency, we used primary CD4+ T cells isolated from HIV+ individuals. As for the primary HIV latency model, resting cells from HIV+ individuals were either not treated or TCR-treated before single cell isolation and single-cell RNA-Seq.
Organism:
Secondary Study Accession:
SRP134967
Study Title:
Single-Cell RNA-Seq Reveals Transcriptional Heterogeneity in Latent and Reactivated HIV-infected Cells
Center Name:
D-BSSE, ETH Zürich
Study Name:
Single-Cell RNA-Seq Reveals Transcriptional Heterogeneity in Latent and Reactivated HIV-infected Cells
ENA-REFSEQ:
N
PROJECT-ID:
437966
ENA-FIRST-PUBLIC:
2018-03-14
ENA-LAST-UPDATE:
2025-02-23
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Homo sapiens | N/ASubmitted files are only available for data submitted via ENA | N/A | |||||||
Homo sapiens | N/ASubmitted files are only available for data submitted via ENA | N/A | |||||||
Homo sapiens | N/ASubmitted files are only available for data submitted via ENA | N/A |
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