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Repository ID:
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FR-FCM-ZYGW
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Experiment name:
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Investigation of the membrane integrity of mycobacteria
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MIFlowCyt score:
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52.63%
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Primary researcher:
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CARINE SAO |
PI/manager:
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CARINE SAO |
Uploaded by:
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CARINE SAO |
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Experiment dates:
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2017-02-01 - 2018-02-01 |
Dataset uploaded:
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Feb 2018
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Last updated:
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Feb 2018
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Keywords:
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[ergothioneine]
[mycothiol]
[gamma-glutamylcysteine]
[mycobacterial membrane]
[thiols secretion]
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Manuscripts:
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[29437626]
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Organizations:
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Stellenbosch University, Biomedical Sciences, Cape Town, Western Cape (South Africa)
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Purpose:
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Demonstrate that the membrane of some Mycobacterium tuberculosis mutants is not more damaged than the membrane of the wild type. Demonstrate that the detected extracellular thiols could be as a result of a secretion. |
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Conclusion:
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The membrane of tested thiol-defcient mutants is not more damaged than that of the wild type strain. Therefore the elevated extracellular thiols could result from a secretion.
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Comments:
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The deficiency of one thiol results leads to an increased intracellular production and extracellular secretion of the other thiols in Mycobacterium tuberculosis.
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Funding:
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The South African Medical Research Council (SAMRC), the DST-NRF Centre of Excellence in Biomedical Tuberculosis Research, the Harry Crossley Foundation and Norvatis (Basel, Switzerland).
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Quality control:
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Samples were washed twice and re-suspended in 150 mM NaCl. |
