|
| Status |
Public on Mar 06, 2019 |
| Title |
Functional Taxonomy of CENPA-Bound Genes in Prostate Cancer |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Overexpression of centromeric proteins has been identified in a number of human malignancies, though their functional and mechanistic contributions to disease progression have not been characterized. CENPA, the centromeric histone H3 variant, is the epigenetic mark that determines centromere identity. Here, we demonstrate that CENPA is highly overexpressed in prostate cancer in both tissue and cell lines, and the level of CENPA expression correlates with the stage of disease. Gain-of- and loss-of-function experimentation confirms that CENPA promotes prostate cancer cell line growth. Integrated sequencing studies further reveal a previously unidentified function of CENPA as a transcriptional regulator that modulates expression of critical proliferation, cell-cycle, and centromere/kinetochore genes. Our findings, therefore, suggest a previously undescribed biological function for CENPA, a protein normally thought to be solely and importantly involved in centromere identity.
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| |
|
| Overall design |
Identification of extracentromeric CENPA binding in prostate cancer using ChIP-seq
|
| |
|
| Contributor(s) |
Qin T, Sartor M, Saha A, Markovitz D |
| Citation(s) |
32371391 |
| Submission date |
Mar 05, 2019 |
| Last update date |
May 21, 2020 |
| Contact name |
Anjan Kumar Saha |
| E-mail(s) |
aksaha@umich.edu
|
| Phone |
7037853087
|
| Organization name |
University of Michigan
|
| Street address |
1150 West Medical Center Drive
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48105 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (6)
|
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| This SubSeries is part of SuperSeries: |
| GSE127837 |
CENPA-Bound Genes and Transcriptional Profiling of CENPA-Depleted Prostate Cancer Cells |
|
| Relations |
| BioProject |
PRJNA525634 |
| SRA |
SRP187531 |
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