|
| Status |
Public on May 05, 2020 |
| Title |
Neuroligin 3 Splice Isoforms Shape Mouse Hippocampal Inhibitory Synaptic Function |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Synapse formation is a dynamic process essential for neuronal circuit development and maturation. At the synaptic cleft, trans-synaptic protein-protein interactions constitute major biological determinants of proper synapse efficacy. The balance of excitatory and inhibitory synaptic transmission (E-I balance) stabilizes synaptic activity and its dysregulation has been implicated in neurodevelopmental disorders including autism spectrum disorders. However, the molecular mechanisms underlying E-I balance remains to be elucidated. Here, we investigate Neuroligin (Nlgn) genes which encode a family of postsynaptic adhesion molecules that shape excitatory and inhibitory synaptic function. We identified that NLGN3 protein differentially regulates inhibitory synaptic transmission in a splice isoform-dependent manner in hippocampal CA1 synapses. Distinct subcellular localization patterns of NLGN3 isoforms contribute to the functional differences observed among splice variants. Finally, our single-cell sequencing analysis reveals that Nlgn1 and Nlgn3 are the major Nlgn genes and that Nlgn splice isoforms are highly diverse in CA1 pyramidal neurons.
|
| |
|
| Overall design |
The cytosol of single cell CA1 neuron was harvested using the whole cell patch-clamp technique
|
| |
|
| Contributor(s) |
Uchigashima M, Leung M, Watanabe T, Cheung A, Watanabe M, Imamura Kawasawa Y, Futai K |
| Citation(s) |
32381505 |
| Submission date |
Jan 08, 2020 |
| Last update date |
Aug 04, 2020 |
| Contact name |
Yuka Imamura Kawasawa |
| E-mail(s) |
yui102@psu.edu
|
| Organization name |
Penn State University
|
| Department |
College of Medicine, Pharmacology
|
| Street address |
500 University Dr.
|
| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA599927 |
| SRA |
SRP240353 |
Less...
More...