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Status |
Public on May 09, 2020 |
Title |
ChIP-seq BHT-101 cells and BHT-101 ASH1L KO cell lines |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
The goal of this study is to assess the role of ASH1 like histone lysine methyltransferase (ASH1L) in the biology of anaplastic thyroid cancer. CRISPR-Cas9 was used to create cell lines derived from BHT-101 anaplastic thyroid cancer cells with premature stop codons prior to the catalytic domain within both alleles of ASH1L. ChIP-seq for H3K36me2, the histone mark catalyzed by ASH1L, was performed on two KO cell lines, and compared to wild type BHT-101 cells.
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Overall design |
H3K36me2 ChIP-seq was performed on wild type BHT-101 cells and 2 BHT-101 ASH1L KO cell lines. Three biological replicates were performed and analyzed.
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Contributor(s) |
Xu B, Qin T, Yu J, Giordano TJ, Sartor MA, Koenig RJ |
Citation(s) |
32398261 |
Submission date |
Mar 16, 2020 |
Last update date |
May 14, 2020 |
Contact name |
Ronald J Koenig |
E-mail(s) |
rkoenig@umich.edu
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Phone |
734-764-9679
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Organization name |
University of Michigan
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Department |
Metabolism, Endocrinology & Diabetes
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Lab |
5560 MSRB-2, SPC 5678
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Street address |
1150 West Medical Center Dr
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City |
Ann Arbor |
State/province |
MI |
ZIP/Postal code |
48109 |
Country |
USA |
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Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (18)
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Relations |
BioProject |
PRJNA612888 |
SRA |
SRP253006 |
Supplementary file |
Size |
Download |
File type/resource |
GSE147074_723_clone3-11_H3K36me2.broadPeak.gz |
722.1 Kb |
(ftp)(http) |
BROADPEAK |
GSE147074_723_clone4-14_H3K36me2.broadPeak.gz |
809.4 Kb |
(ftp)(http) |
BROADPEAK |
GSE147074_801_BHT101wt_H3K36me2.broadPeak.gz |
1.8 Mb |
(ftp)(http) |
BROADPEAK |
GSE147074_801_clone3-11_H3K36me2.broadPeak.gz |
1.5 Mb |
(ftp)(http) |
BROADPEAK |
GSE147074_801_clone4-14_H3K36me2.broadPeak.gz |
1.4 Mb |
(ftp)(http) |
BROADPEAK |
GSE147074_820_BHT101wt_H3K36me2.broadPeak.gz |
176.9 Kb |
(ftp)(http) |
BROADPEAK |
GSE147074_820_clone3-11_H3K36me2.broadPeak.gz |
304.7 Kb |
(ftp)(http) |
BROADPEAK |
GSE147074_820_clone4-14_H3K36me2.broadPeak.gz |
1.7 Mb |
(ftp)(http) |
BROADPEAK |
GSE147074_H3K36me2_BHT101.broadPeak.gz |
750.5 Kb |
(ftp)(http) |
BROADPEAK |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |