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Series GSE147074 Query DataSets for GSE147074
Status Public on May 09, 2020
Title ChIP-seq BHT-101 cells and BHT-101 ASH1L KO cell lines
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The goal of this study is to assess the role of ASH1 like histone lysine methyltransferase (ASH1L) in the biology of anaplastic thyroid cancer. CRISPR-Cas9 was used to create cell lines derived from BHT-101 anaplastic thyroid cancer cells with premature stop codons prior to the catalytic domain within both alleles of ASH1L. ChIP-seq for H3K36me2, the histone mark catalyzed by ASH1L, was performed on two KO cell lines, and compared to wild type BHT-101 cells.
 
Overall design H3K36me2 ChIP-seq was performed on wild type BHT-101 cells and 2 BHT-101 ASH1L KO cell lines. Three biological replicates were performed and analyzed.
 
Contributor(s) Xu B, Qin T, Yu J, Giordano TJ, Sartor MA, Koenig RJ
Citation(s) 32398261
Submission date Mar 16, 2020
Last update date May 14, 2020
Contact name Ronald J Koenig
E-mail(s) rkoenig@umich.edu
Phone 734-764-9679
Organization name University of Michigan
Department Metabolism, Endocrinology & Diabetes
Lab 5560 MSRB-2, SPC 5678
Street address 1150 West Medical Center Dr
City Ann Arbor
State/province MI
ZIP/Postal code 48109
Country USA
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (18)
GSM4416458 Expt1_IP_WT
GSM4416459 Expt1_Input-WT
GSM4416460 Expt1_IP_KO clone 1
Relations
BioProject PRJNA612888
SRA SRP253006

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE147074_723_clone3-11_H3K36me2.broadPeak.gz 722.1 Kb (ftp)(http) BROADPEAK
GSE147074_723_clone4-14_H3K36me2.broadPeak.gz 809.4 Kb (ftp)(http) BROADPEAK
GSE147074_801_BHT101wt_H3K36me2.broadPeak.gz 1.8 Mb (ftp)(http) BROADPEAK
GSE147074_801_clone3-11_H3K36me2.broadPeak.gz 1.5 Mb (ftp)(http) BROADPEAK
GSE147074_801_clone4-14_H3K36me2.broadPeak.gz 1.4 Mb (ftp)(http) BROADPEAK
GSE147074_820_BHT101wt_H3K36me2.broadPeak.gz 176.9 Kb (ftp)(http) BROADPEAK
GSE147074_820_clone3-11_H3K36me2.broadPeak.gz 304.7 Kb (ftp)(http) BROADPEAK
GSE147074_820_clone4-14_H3K36me2.broadPeak.gz 1.7 Mb (ftp)(http) BROADPEAK
GSE147074_H3K36me2_BHT101.broadPeak.gz 750.5 Kb (ftp)(http) BROADPEAK
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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