|
| Status |
Public on May 09, 2020 |
| Title |
RNA-seq BHT-101 cells and BHT-101 ASH1L KO cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study is to assess the role of ASH1 like histone lysine methyltransferase (ASH1L) in the biology of anaplastic thyroid cancer. CRISPR-Cas9 was used to create 4 independent cell lines derived from BHT-101 anaplastic thyroid cancer cells with premature stop codons prior to the catalytic domain within both alleles of ASH1L. RNA-seq was performed on these 4 KO cell lines, and compared to 3 biological replicates of wild type BHT-101 cells.
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| |
|
| Overall design |
3 biological replicates of wild type BHT-101 cells were compared to singlicates of the 4 ASH1L KO cell lines
|
| |
|
| Contributor(s) |
Xu B, Qin T, Yu J, Giordano TJ, Sartor MA, Koenig RJ |
| Citation(s) |
32398261 |
| Submission date |
Mar 16, 2020 |
| Last update date |
May 14, 2020 |
| Contact name |
Ronald J Koenig |
| E-mail(s) |
rkoenig@umich.edu
|
| Phone |
734-764-9679
|
| Organization name |
University of Michigan
|
| Department |
Metabolism, Endocrinology & Diabetes
|
| Lab |
5560 MSRB-2, SPC 5678
|
| Street address |
1150 West Medical Center Dr
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (7)
|
|
| Relations |
| BioProject |
PRJNA612886 |
| SRA |
SRP253008 |
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