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| Status |
Public on Mar 03, 2022 |
| Title |
mRNA expression profiles of murine macrophages (cell line: RAW264.7) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the transcriptome profile of murine macrophages (cell line: RAW264.7) after supplementation with polyunsaturated fatty acids (PUFA) and stimulation with LTA. The fatty acids docosahexaenoic acid (DHA, C22:6n3) or arachidonic acid (AA, C20:4n6) were included in the culture medium in concentrations of 15 µmol/L using ethanol as a vehicle (0.2 % v/v final ethanol concentration). Cells were cultured in the enriched media totaling 72 h. Stimulation of cells was performed in the last 24 h of fatty acid supplementation by addition of LTA (0.5 µg/mL; from Staphylococcus aureus).
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| Overall design |
mRNA profiles of murine macrophages (cell line: RAW264.7) supplemented with unsaturated fatty acids and/or exposed to an inflammatory milieu were generated by deep sequencing, in triplicate, using Illumina sequencing.
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| Contributor(s) |
Schumann J, Roessler C |
| Citation(s) |
35205256, 37301912 |
| Submission date |
Dec 10, 2020 |
| Last update date |
Sep 08, 2023 |
| Contact name |
Julia Schumann |
| E-mail(s) |
julia.schumann@uk-halle.de
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| Organization name |
University Medicine Halle (Saale)
|
| Street address |
Franzosenweg 1a
|
| City |
Halle (Saale) |
| ZIP/Postal code |
06112 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE162997 |
mRNA and miRNA expression profiles of murine macrophages (cell line: RAW264.7) |
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| Relations |
| BioProject |
PRJNA684304 |
| SRA |
SRP299563 |
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