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Status |
Public on Mar 03, 2022 |
Title |
miRNA expression profiles of murine macrophages (cell line: RAW264.7) |
Organism |
Mus musculus |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
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Summary |
We report the miRNA profile of murine macrophages (cell line: RAW264.7) after supplementation with polyunsaturated fatty acids (PUFA) and stimulation with LTA. The fatty acids docosahexaenoic acid (DHA, C22:6n3) or arachidonic acid (AA, C20:4n6) were included in the culture medium in concentrations of 15 µmol/L using ethanol as a vehicle (0.2 % v/v final ethanol concentration). Cells were cultured in the enriched media totaling 72 h. Stimulation of cells was performed in the last 24 h of fatty acid supplementation by addition of LTA (0.5 µg/mL; from Staphylococcus aureus).
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Overall design |
miRNA profiles of murine macrophages (cell line: RAW264.7) supplemented with unsaturated fatty acids and/or exposed to an inflammatory milieu were generated by deep sequencing, in triplicate, using Illumina sequencing.
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Contributor(s) |
Schumann J, Roessler C |
Citation(s) |
35205256, 37301912 |
Submission date |
Dec 10, 2020 |
Last update date |
Sep 08, 2023 |
Contact name |
Julia Schumann |
E-mail(s) |
julia.schumann@uk-halle.de
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Organization name |
University Medicine Halle (Saale)
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Street address |
Franzosenweg 1a
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City |
Halle (Saale) |
ZIP/Postal code |
06112 |
Country |
Germany |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (12)
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This SubSeries is part of SuperSeries: |
GSE162997 |
mRNA and miRNA expression profiles of murine macrophages (cell line: RAW264.7) |
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Relations |
BioProject |
PRJNA684306 |
SRA |
SRP297571 |