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| Status |
Public on Apr 30, 2022 |
| Title |
Single cell profiling of primary and paired metastatic lymph node tumors in breast cancer patients(10x RNA and TCR) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The microenvironment of lymph node metastasized tumors (LNMT) determines tumor progression and response to therapy, but a systematic study of LNMT is lacking. Here, we generate single-cell maps of primary tumors (PTs) and paired LNMTs in 8 breast cancer patients. We demonstrate that the activation, cytotoxicity, and proliferation of T cells are suppressed in LNMT compared with PT. CD4+CXCL13+ T cells in LNMT are more likely to differentiate into an exhausted state. Interestingly, LAMP3+ dendritic cells in LNMT display lower T cell priming and activating ability than in PT. Additionally, we identify a subtype of PLA2G2A+ cancer-associated fibroblasts enriched in HER2+ breast cancer patients that promotes immune infiltration. We also show that the antigen-presentation pathway is downregulated in malignant cells of the metastatic lymph node. Altogether, we characterize the microenvironment of LNMT and PT, which may shed light on the individualized therapeutic strategies for breast cancer patients with lymph node metastasis.
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| |
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| Overall design |
Single cell RNA and TCR sequencing of primary tumor and paired lymph node metastasis in breast cancer
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| |
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| Contributor(s) |
Liu T, Liu C, Zhang J, Wei X, Zhang H |
| Citation(s) |
36357424 |
| Submission date |
Feb 18, 2021 |
| Last update date |
Dec 29, 2022 |
| Contact name |
Hongquan Zhang |
| E-mail(s) |
hongquan.zhang@bjmu.edu.cn
|
| Organization name |
Peking University
|
| Department |
Department of Anatomy and embryology
|
| Street address |
School of Basic Medical Sciences. Peking University Health Science Center NO. 38, XUEYUAN Road
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100191 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (32)
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| This SubSeries is part of SuperSeries: |
| GSE190870 |
Single cell profiling of primary and paired metastatic lymph node tumors in breast cancer patients |
|
| Relations |
| BioProject |
PRJNA702767 |
| SRA |
SRP306993 |
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