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| Status |
Public on Jul 20, 2022 |
| Title |
tsRNAs Are Potential Biomarkers of Acute Rejection in Vascularized Composite Allotransplantation |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: to explore the function and mechanism of acute rejection in vascularized composite allotransplantation
Method: tRF & tiRNA profiles of acute rejection in vascularized composite allotransplantation were generated by deep sequencing, using Illumina NextSeq 500. qRT–PCR validation was performed using SYBR Green assays.
Results:Sequencing was used to screen expression profiles and predict target genes. Compared with the control group, there were significant differences in the expression levels of 16 tRFs & tiRNAs, including 5 up-regulated target genes and 11 down-regulated target genes. Bioinformatics analysis and RT-qPCR revealed potential up regulation of tRFs & tiRNAs(tiRNA-1-33-Gly-GCC-1, tiRNA-1-34-Glu-CTC-1, tRF-1-32-Gly-GCC-2-M2) and down regulation of tRFs & tiRNAs (tRF-59:75-Gln-CTG-2-M2, tRF-59:76-Val-AAC-1-M2).
Conclusion: In conclusion, it is speculated that tiRNA-1-34-Glu-CTC-1 plays an important role in VCA induced acute rejection by regulating CACNA1D gene in MAPK signaling pathway. This provides a new insight into the mechanism and therapeutic targets of acute rejection.
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| Overall design |
tRF & tiRNA profiles of normal skin and UV damage skin mice
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| Contributor(s) |
Fang Y, Wang S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 02, 2021 |
| Last update date |
Jul 24, 2022 |
| Contact name |
yuan fang |
| E-mail(s) |
jingji5996@gmail.com
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| Phone |
15273191040
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| Organization name |
The Third Xiangya Hospital of Central South Uniiversity
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| Street address |
138 TongZiPo road
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| City |
chang sha |
| ZIP/Postal code |
410013 |
| Country |
China |
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| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA785574 |
| SRA |
SRP348911 |
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