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Series GSE202381 Query DataSets for GSE202381
Status Public on May 13, 2022
Title Gonadectomy-induced transcriptome changes in the ventral cervical spinal cord of male and female rats
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary Circulating sex steroids are critical for the development of neuroplasticity in the respiratory motor system. A primary locus of inducible respiratory neuroplasticity is the phrenic motor nucleus, a column of motor neurons in the ventral cervical spinal cord (C3-C5). In specific, exposure to acute intermittent hypoxia (AIH) induces strengthened synaptic connections to phrenic motor neurons and increased motor output; a form of neuroplasticity called phrenic long-term facilitation (pLTF). Gonadectomy (surgical removal of the testes in males and ovaries in females) reduces circulating sex steroids and eliminates AIH-induced phrenic pLTF in both females and males, implicating sex steroids as critical mediators for the development of neuroplasticity. To begin interrogating mechanisms of sex steroid influence on phrenic motor neurons and to determine the effect of gonadectomy on local gene expression, we performed RNA sequencing on ventral cervical spinal cord samples containing the phrenic motor nucleus. Our findings identified sex-specific gene expression in gonadally-intact rats and suggested that gonadectomy caused greater changes to the transcriptomes of male rats compared to females.
 
Overall design Comparative gene expression profiling analysis of RNA-seq data for ventral cervical spinal cord tissue (C3-C6) of female and male rats with and without gonadectomy.
 
Contributor(s) Miller S, Abrahante JE, Roopra A, Dougherty BJ
Citation(s) 36581616
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 HL146477 Estrogen Receptor Signaling in the Expression of Respiratory Motor Plasticity UNIVERSITY OF MINNESOTA Brendan J Dougherty
Submission date May 06, 2022
Last update date Jan 02, 2023
Contact name Brendan J. Dougherty
E-mail(s) bdougher@UMN.edu
Phone 6126247466
Organization name University of Minnesota - Twin Cities
Department Rehabilitation Medicine
Street address 420 Delaware Street SE, MMC 388
City MINNEAPOLIS
State/province MN
ZIP/Postal code 55455
Country USA
 
Platforms (1)
GPL20084 Illumina NextSeq 500 (Rattus norvegicus)
Samples (22)
GSM6112658 Ventral spinal cord, C3-C6, intact male, Rat 1
GSM6112659 Ventral spinal cord, C3-C6, intact male, Rat 2
GSM6112660 Ventral spinal cord, C3-C6, intact male, Rat 3
Relations
BioProject PRJNA835823

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Supplementary file Size Download File type/resource
GSE202381_RawReadCounts_Dougherty002.txt.gz 917.2 Kb (ftp)(http) TXT
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