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Status |
Public on May 13, 2022 |
Title |
Gonadectomy-induced transcriptome changes in the ventral cervical spinal cord of male and female rats |
Organism |
Rattus norvegicus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Circulating sex steroids are critical for the development of neuroplasticity in the respiratory motor system. A primary locus of inducible respiratory neuroplasticity is the phrenic motor nucleus, a column of motor neurons in the ventral cervical spinal cord (C3-C5). In specific, exposure to acute intermittent hypoxia (AIH) induces strengthened synaptic connections to phrenic motor neurons and increased motor output; a form of neuroplasticity called phrenic long-term facilitation (pLTF). Gonadectomy (surgical removal of the testes in males and ovaries in females) reduces circulating sex steroids and eliminates AIH-induced phrenic pLTF in both females and males, implicating sex steroids as critical mediators for the development of neuroplasticity. To begin interrogating mechanisms of sex steroid influence on phrenic motor neurons and to determine the effect of gonadectomy on local gene expression, we performed RNA sequencing on ventral cervical spinal cord samples containing the phrenic motor nucleus. Our findings identified sex-specific gene expression in gonadally-intact rats and suggested that gonadectomy caused greater changes to the transcriptomes of male rats compared to females.
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Overall design |
Comparative gene expression profiling analysis of RNA-seq data for ventral cervical spinal cord tissue (C3-C6) of female and male rats with and without gonadectomy.
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Contributor(s) |
Miller S, Abrahante JE, Roopra A, Dougherty BJ |
Citation(s) |
36581616 |
NIH grant(s) |
Grant ID |
Grant title |
Affiliation |
Name |
R01 HL146477 |
Estrogen Receptor Signaling in the Expression of Respiratory Motor Plasticity |
UNIVERSITY OF MINNESOTA |
Brendan J Dougherty |
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Submission date |
May 06, 2022 |
Last update date |
Jan 02, 2023 |
Contact name |
Brendan J. Dougherty |
E-mail(s) |
bdougher@UMN.edu
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Phone |
6126247466
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Organization name |
University of Minnesota - Twin Cities
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Department |
Rehabilitation Medicine
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Street address |
420 Delaware Street SE, MMC 388
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City |
MINNEAPOLIS |
State/province |
MN |
ZIP/Postal code |
55455 |
Country |
USA |
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Platforms (1) |
GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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Samples (22)
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GSM6112658 |
Ventral spinal cord, C3-C6, intact male, Rat 1 |
GSM6112659 |
Ventral spinal cord, C3-C6, intact male, Rat 2 |
GSM6112660 |
Ventral spinal cord, C3-C6, intact male, Rat 3 |
GSM6112661 |
Ventral spinal cord, C3-C6, intact male, Rat 5 |
GSM6112662 |
Ventral spinal cord, C3-C6, intact male, Rat 6 |
GSM6112663 |
Ventral spinal cord, C3-C6, castrated male, Rat 1 |
GSM6112664 |
Ventral spinal cord, C3-C6, castrated male, Rat 2 |
GSM6112665 |
Ventral spinal cord, C3-C6, castrated male, Rat 4 |
GSM6112666 |
Ventral spinal cord, C3-C6, castrated male, Rat 5 |
GSM6112667 |
Ventral spinal cord, C3-C6, castrated male, Rat 6 |
GSM6112668 |
Ventral spinal cord, C3-C6, intact female, Rat 1 |
GSM6112669 |
Ventral spinal cord, C3-C6, intact female, Rat 2 |
GSM6112670 |
Ventral spinal cord, C3-C6, intact female, Rat 3 |
GSM6112671 |
Ventral spinal cord, C3-C6, intact female, Rat 4 |
GSM6112672 |
Ventral spinal cord, C3-C6, intact female, Rat 5 |
GSM6112673 |
Ventral spinal cord, C3-C6, intact female, Rat 6 |
GSM6112674 |
Ventral spinal cord, C3-C6, Ovariectomized female, Rat 1 |
GSM6112675 |
Ventral spinal cord, C3-C6, Ovariectomized female, Rat 2 |
GSM6112676 |
Ventral spinal cord, C3-C6, Ovariectomized female, Rat 3 |
GSM6112677 |
Ventral spinal cord, C3-C6, Ovariectomized female, Rat 4 |
GSM6112678 |
Ventral spinal cord, C3-C6, Ovariectomized female, Rat 5 |
GSM6112679 |
Ventral spinal cord, C3-C6, Ovariectomized female, Rat 6 |
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Relations |
BioProject |
PRJNA835823 |
Supplementary file |
Size |
Download |
File type/resource |
GSE202381_RawReadCounts_Dougherty002.txt.gz |
917.2 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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