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| Status |
Public on May 14, 2023 |
| Title |
Gene expression profile at single cell level of human Simpson Golabi Behmel Syndrome (SGBS) and murine 3T3-L1 cells prior to and during adipogenesis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We used single cell RNA sequencing (scRNA-seq) to analyze the transcriptional diversity of cells undergoing adipogenesis using two cellular models of adipogenesis in vitro, human SGBS cells and murine 3T3-L1 cells.
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| Overall design |
SGBS and 3T3-L1 cells were isolated at two timepoints: prior to the addition of differentiation stimuli (D0) and during adipogenesis (D5 for 3T3-L1, D8 for SGBS). Live cells were isolated using Fluorescence-activated cell sorting (FACS) according to the absence of staining with propidium iodide. Next, equal numbers of SGBS and 3T3-L1 cells were mixed and analyzed using scRNA-Seq.
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| Contributor(s) |
Knowles JW, Bielczyk-Maczynska E, Li J, Jin C |
| Citation(s) |
37328521 |
| Submission date |
Mar 01, 2023 |
| Last update date |
Aug 13, 2023 |
| Contact name |
Christopher Ann Jin |
| E-mail(s) |
christopherjin@gmail.com
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| Phone |
4086563606
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| Organization name |
Stanford Medical School
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| Department |
Genetics
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| Lab |
Knowles, Snyder
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| Street address |
3165 Porter Drive
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| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94134 |
| Country |
USA |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA940071 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE226365_Final_day_CellRangerQC.html.gz |
882.8 Kb |
(ftp)(http) |
HTML |
| GSE226365_First_day_CellRangerQC.html.gz |
917.3 Kb |
(ftp)(http) |
HTML |
| GSE226365_RAW.tar |
159.9 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
| GSE226365_current_data.human.Rds.gz |
1.2 Gb |
(ftp)(http) |
RDS |
| GSE226365_current_data.markers.human.csv.gz |
72.7 Kb |
(ftp)(http) |
CSV |
| GSE226365_current_data.markers.mouse.csv.gz |
105.2 Kb |
(ftp)(http) |
CSV |
| GSE226365_current_data.mouse.Rds.gz |
984.1 Mb |
(ftp)(http) |
RDS |
| GSE226365_jiehan_scRNAseq_qc_human.html.gz |
2.7 Mb |
(ftp)(http) |
HTML |
| GSE226365_jiehan_scRNAseq_qc_mouse.html.gz |
2.7 Mb |
(ftp)(http) |
HTML |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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