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| Status |
Public on Nov 07, 2023 |
| Title |
Transcriptome analysis of the mouse fetal and adult rete ovarii and surrounding tissues |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The rete ovarii (RO) is an epithelial structure that arises during fetal development in close proximity to the ovary, and persists throughout adulthood in mammals. However, the functional significance of the RO remains elusive and it has been absent from recent discussions of female reproductive anatomy. The RO comprises three distinct regions: the intraovarian rete (IOR) within the ovary, the extraovarian rete (EOR) in the periovarian tissue, and the connecting rete (CR) linking the EOR and IOR. We hypothesize that the RO plays a pivotal role in maintaining ovarian homeostasis and responding to physiological changes. To uncover the nature and function of RO cells, we conducted transcriptome analysis, encompassing bulk, single-cell, and nucleus-level sequencing of both fetal and adult RO tissues using the Pax8-rtTA; Tre-H2B-GFP mouse line, where all RO regions express nuclear GFP. This study presents three datasets, which highlight RO-specific gene expression signatures and reveal differences in gene expression across the three RO regions during development and in adulthood. The integration and rigorous validation of these datasets will advance our understanding of the RO's roles in ovarian development, female maturation, and adult female fertility.
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| Overall design |
This study employs comprehensive bulk, single cell and single nucleus transcriptome analysis to uncover gene expression signatures of the fetal and adult rete ovarii (RO) in mice.
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| Web link |
https://www.biorxiv.org/content/10.1101/2023.11.06.565717v1
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| |
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| Contributor(s) |
Anbarci DN, O'Rourke R, Capel B, McKey J |
| Citation(s) |
38615064 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| K99 HD103778 |
Functions of the rete ovarii in ovary development, adult homeostasis, and female reproductive longevity |
DUKE UNIVERSITY |
Jennifer C McKey |
| R00 HD103778 |
Functions of the rete ovarii in ovary development, adult homeostasis, and female reproductive longevity |
UNIVERSITY OF COLORADO DENVER |
Jennifer C McKey |
|
| Submission date |
Oct 06, 2023 |
| Last update date |
May 01, 2024 |
| Contact name |
Jennifer McKey |
| E-mail(s) |
jennifer.mckey@cuanschutz.edu
|
| Organization name |
CU Anschutz medical campus
|
| Street address |
1775 Aurora Ct Barbara Davis Center, M20-3202G
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA1025006 |
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